Expressing:Article Title: Bispecific antigen binding molecules that bind HER2, and methods of use thereof
Article Snippet: .. TABLE 7 Cell Lines and Relative HER2 Expression Relative HER2 Cell Line Source Catalog # Expression Primary Myocardial Promo C12811 6% cells Cell Primary Prostate ATCC PCS-440-010 1% Epithelial cells Primary Lung ATCC PCS-300-010 1% Epithelial cells Primary Mammary ATCC PCS-600-010 1% Epithelial cells MDA-MB468 ATCC HTB-132 0% MDA-MB231 ATCC HTB-26 3% HepG2 ATCC HB-8065 13% EBC-1 6% (Lung Carcinoma) MCF7 ATCC HTB-22 15% T47D ATCC HTB-133 24% BT483 ATCC HTB121 37% ZR751 ATCC CRL-1500 39% JIMT1 DSMZ ACC589 52% MDA-MB361 ATCC HTB-27 82% MDA-MB453 ATCC HTB-131 60% SK-BR-3 ATCC HTB-30 100% B1474 ATCC HTB-20 89% N87 (NCI-N87) ATCC HTB-5822 93% Calu-3 ATCC HTB-55 84% SKOV-3 ATCC HTB-77 93% Cells were plated on collagen-coated, black wall, 96-well, optical plates (Greiner, Cat #655936) at 2.5×105 cells per well in their appropriate culture media and incubated overnight at 37° C. in 5% CO2. .. The following day, cell were incubated for 30 minutes with 10 ug/mL of Alexa647 labeled (Thermo, Cat #A37573) antibodies in 50 uL of DMEM at 4° C. After, cells were washed twice with cold DMEM+10% FBS and incubated with Δ % paraformaldehyde (Electron Microcopy Sciences, Cat #15710)+0.075% Saponin (Sigma, Cat #S4521)+10 ug/ml Hoechst (Lifetech, #H3569) in PBS for 10 minutes at room temperature.
Multiple Displacement Amplification:Article Title: Bispecific antigen binding molecules that bind HER2, and methods of use thereof
Article Snippet: .. TABLE 7 Cell Lines and Relative HER2 Expression Relative HER2 Cell Line Source Catalog # Expression Primary Myocardial Promo C12811 6% cells Cell Primary Prostate ATCC PCS-440-010 1% Epithelial cells Primary Lung ATCC PCS-300-010 1% Epithelial cells Primary Mammary ATCC PCS-600-010 1% Epithelial cells MDA-MB468 ATCC HTB-132 0% MDA-MB231 ATCC HTB-26 3% HepG2 ATCC HB-8065 13% EBC-1 6% (Lung Carcinoma) MCF7 ATCC HTB-22 15% T47D ATCC HTB-133 24% BT483 ATCC HTB121 37% ZR751 ATCC CRL-1500 39% JIMT1 DSMZ ACC589 52% MDA-MB361 ATCC HTB-27 82% MDA-MB453 ATCC HTB-131 60% SK-BR-3 ATCC HTB-30 100% B1474 ATCC HTB-20 89% N87 (NCI-N87) ATCC HTB-5822 93% Calu-3 ATCC HTB-55 84% SKOV-3 ATCC HTB-77 93% Cells were plated on collagen-coated, black wall, 96-well, optical plates (Greiner, Cat #655936) at 2.5×105 cells per well in their appropriate culture media and incubated overnight at 37° C. in 5% CO2. .. The following day, cell were incubated for 30 minutes with 10 ug/mL of Alexa647 labeled (Thermo, Cat #A37573) antibodies in 50 uL of DMEM at 4° C. After, cells were washed twice with cold DMEM+10% FBS and incubated with Δ % paraformaldehyde (Electron Microcopy Sciences, Cat #15710)+0.075% Saponin (Sigma, Cat #S4521)+10 ug/ml Hoechst (Lifetech, #H3569) in PBS for 10 minutes at room temperature.
Incubation:Article Title: Bispecific antigen binding molecules that bind HER2, and methods of use thereof
Article Snippet: .. TABLE 7 Cell Lines and Relative HER2 Expression Relative HER2 Cell Line Source Catalog # Expression Primary Myocardial Promo C12811 6% cells Cell Primary Prostate ATCC PCS-440-010 1% Epithelial cells Primary Lung ATCC PCS-300-010 1% Epithelial cells Primary Mammary ATCC PCS-600-010 1% Epithelial cells MDA-MB468 ATCC HTB-132 0% MDA-MB231 ATCC HTB-26 3% HepG2 ATCC HB-8065 13% EBC-1 6% (Lung Carcinoma) MCF7 ATCC HTB-22 15% T47D ATCC HTB-133 24% BT483 ATCC HTB121 37% ZR751 ATCC CRL-1500 39% JIMT1 DSMZ ACC589 52% MDA-MB361 ATCC HTB-27 82% MDA-MB453 ATCC HTB-131 60% SK-BR-3 ATCC HTB-30 100% B1474 ATCC HTB-20 89% N87 (NCI-N87) ATCC HTB-5822 93% Calu-3 ATCC HTB-55 84% SKOV-3 ATCC HTB-77 93% Cells were plated on collagen-coated, black wall, 96-well, optical plates (Greiner, Cat #655936) at 2.5×105 cells per well in their appropriate culture media and incubated overnight at 37° C. in 5% CO2. .. The following day, cell were incubated for 30 minutes with 10 ug/mL of Alexa647 labeled (Thermo, Cat #A37573) antibodies in 50 uL of DMEM at 4° C. After, cells were washed twice with cold DMEM+10% FBS and incubated with Δ % paraformaldehyde (Electron Microcopy Sciences, Cat #15710)+0.075% Saponin (Sigma, Cat #S4521)+10 ug/ml Hoechst (Lifetech, #H3569) in PBS for 10 minutes at room temperature.
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